Intratumor dihydropyrimidine dehydrogenase mRNA expression levels are decreased in extramammary Paget's disease

Yuki Nakamura; Ikko Kajihara; Saori Yamada-Kanazawa; Saki Maeda-Otsuka; Takamitsu Johno; Jun Aoi; Toshikatsu Igata; Shinichi Masuguchi; Satoshi Fukushima; Masatoshi Jinnin; Hironobu Ihn

doi:10.5582/ddt.2017.01022

Brief Reports

Intratumor dihydropyrimidine dehydrogenase mRNA expression levels are decreased in extramammary Paget's disease

Yuki Nakamura, Ikko Kajihara, Saori Yamada-Kanazawa, Saki Maeda-Otsuka, Takamitsu Johno, Jun Aoi, Toshikatsu Igata, Shinichi Masuguchi, Satoshi Fukushima, Masatoshi Jinnin, Hironobu Ihn

Author information

Yuki Nakamura
Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University
Ikko Kajihara
Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University
Saori Yamada-Kanazawa
Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University
Saki Maeda-Otsuka
Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University
Takamitsu Johno
Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University
Jun Aoi
Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University
Toshikatsu Igata
Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University
Shinichi Masuguchi
Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University
Satoshi Fukushima
Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University
Masatoshi Jinnin
Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University
Hironobu Ihn
Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University

Keywords: S-1, extramammary Paget's disease, mRNA, real time PCR, thymidylate synthase, orotate phosphoribosyl-transferase, dihydropyrimidine dehydrogenase

JOURNAL FREE ACCESS

2017 Volume 11 Issue 3 Pages 152-155

DOI https://doi.org/10.5582/ddt.2017.01022

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Abstract

S-1, a 5-fluorouracil (5-FU)-based anti-cancer agent, is an important drug for treating metastatic extramammary Paget's disease (EMPD). Although intratumor expression levels of 5-FU metabolism enzymes have been studied widely in many solid tumors, no studies have examined on the expression levels of thymidylate synthase (TS), orotate phosphoribosyl-transferase (OPRT) or dihydropyrimidine dehydrogenase (DPD) in skin cancers. The aim of this study was to estimate the intratumoral mRNA expression levels of these genes in EMPD by real time PCR. Intratumoral DPD mRNA levels were decreased in EMPD compared to those in normal skin, but its intratumoral DPD mRNA expression levels were not correlated with clinical manifestations. Intratumoral DPD mRNA levels were positively correlated with OPRT mRNA levels in EMPD. Based on these results, low expression of intratumoral DPD mRNA in EMPD may contribute to the pathogenesis of this disease.

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