Detection of Monoclonal Hematopoiesis Using Restriction Fragment Length Polymorphism (RFLP) of Phosphoglycerokinase (PGK) Gene in a Patient with Hypoplastic Myelodysplastic Syndrome (MDS)

Yasushi MIYAZAKI; Kazutaka KURIYAMA; Tsuneo FUJWARA; Itsuro JINNAI; Kunitake HIRASHIMA; Masao TOMONAGA

doi:10.2169/internalmedicine.32.255

Yasushi MIYAZAKI, Kazutaka KURIYAMA, Tsuneo FUJWARA, Itsuro JINNAI, Kunitake HIRASHIMA, Masao TOMONAGA

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Keywords: diagnosis of MDS, PGK gene analysis

JOURNAL FREE ACCESS

1993 Volume 32 Issue 3 Pages 255-256

DOI https://doi.org/10.2169/internalmedicine.32.255

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Abstract

A clonal analysis of hematopoiesis in a hypoplastic MDS patient was performed. Monoclonal utilization of PGK gene on her two X chromosomes was detected by Southern blot analysis of the bone marrow cells using a PGK probe after methylation-sensitive restriction enzyme treatment. This is compatible with monoclonal construction of her hematopoiesis, confirming MDS nature with a progression of her morphological dysplasia in bone marrow cells. RFLP of PGK gene on X chromosome is thus useful to detect a monoclonality in hematopoietic diseases.
(Internal Medicine 32: 255-256, 1993)

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