Proteome analysis of silkworm 3. Malpighian tube

Hideyuki Kajiwara; Atsue Imamaki; Masatoshi Nakamura; Kazuei Mita; Qingyu Xia; Masami Ishizaka

doi:10.2198/jelectroph.53.33

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Proteome analysis of silkworm 3. Malpighian tube

Hideyuki Kajiwara, Atsue Imamaki, Masatoshi Nakamura, Kazuei Mita, Qingyu Xia, Masami Ishizaka

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Keywords: Malpighian tube, mass spectrometry, proteome, silkworm (Bombyx mori L.), two-dimensional polyacrylamide gel electrophoresis

JOURNAL FREE ACCESS

2009 Volume 53 Issue 2 Pages 33-38

DOI https://doi.org/10.2198/jelectroph.53.33

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Abstract

Proteins in the Malpighian tubes of silkworms were proteomically analyzed by combination of two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. Several proteins were identified using peptide mass fingerprinting by mass spectrometry and subsequent MS/MS analysis. In this analysis, the electrophoresis patterns of the Malpighian tubes were compared from the fourth-instar day-1 larva stage to the stage before adult moth. The results of mass spectrometry analysis and changes in gel images are summarized in the silkworm proteome database (http://kaiko2ddb.dna.affrc.go.jp/cgi-bin/search_2DDB.cgi).

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