Effect of a chitosan sponge impregnated with platelet-derived growth factor on bone augmentation beyond the skeletal envelope in rat calvaria

Noriko Tsuchiya; Shuichi Sato; Risa Kigami; Eisuke Kawano; Masatoshi Takane; Yoshinori Arai; Koichi Ito; Bunnai Ogiso

doi:10.2334/josnusd.56.23

Original

Effect of a chitosan sponge impregnated with platelet-derived growth factor on bone augmentation beyond the skeletal envelope in rat calvaria

Noriko Tsuchiya, Shuichi Sato, Risa Kigami, Eisuke Kawano, Masatoshi Takane, Yoshinori Arai, Koichi Ito, Bunnai Ogiso

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Noriko Tsuchiya
Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry
Shuichi Sato
Department of Periodontology, Nihon University School of Dentistry
Division of Advanced Dental Treatment, Dental Research Center, Nihon University School of Dentistry
Risa Kigami
Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry
Eisuke Kawano
Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry
Masatoshi Takane
Department of Periodontology, Nihon University School of Dentistry
Yoshinori Arai
Nihon University School of Dentistry
Koichi Ito
Nihon University School of Dentistry
Bunnai Ogiso
Department of Periodontology, Nihon University School of Dentistry
Division of Advanced Dental Treatment, Dental Research Center, Nihon University School of Dentistry
Department of Endodontics, Nihon University School of Dentistry

Keywords: PDGF, bone regeneration, rat, chitosan, micro-CT

JOURNAL FREE ACCESS

2014 Volume 56 Issue 1 Pages 23-28

DOI https://doi.org/10.2334/josnusd.56.23

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Abstract

We evaluated the ability of platelet-derived growth factor (PDGF) to promote bone augmentation beyond the skeletal envelope in rat calvaria. The calvariae of 14 rats were exposed, and two plastic caps–one with 0.03% PDGF and a chitosan sponge and one with a chitosan sponge alone–were placed. Microcomputed tomography and histologic sections were used to determine the amount of bone augmentation within the plastic caps. Bone volume was calculated using measurement software. Bone volume and amount of bone augmentation were significantly greater in the PDGF group than in the control group. In conclusion, a chitosan sponge containing 0.03% PDGF enhanced bone formation beyond the skeletal envelope in rat calvaria. (J Oral Sci 56, 23-28, 2014)

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