Importance of the Porcine ADAM3 Disintegrin Domain in Sperm-Egg Interaction

Ekyune KIM; Ki-Eun PARK; Ji-Su KIM; Dong Chul BAEK; Jae-Woong LEE; Sang-Rae LEE; Myeong-Su KIM; Sang-Hyun KIM; Chan-Shick KIM; Deog-Bon KOO; Han-Seok KANG; Zae-Young RYOO; Kyu-Tae CHANG

doi:10.1262/jrd.20134

Full Paper

Importance of the Porcine ADAM3 Disintegrin Domain in Sperm-Egg Interaction

Ekyune KIM, Ki-Eun PARK, Ji-Su KIM, Dong Chul BAEK, Jae-Woong LEE, Sang-Rae LEE, Myeong-Su KIM, Sang-Hyun KIM, Chan-Shick KIM, Deog-Bon KOO, Han-Seok KANG, Zae-Young RYOO, Kyu-Tae CHANG

Author information

Ekyune KIM
National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology
Ki-Eun PARK
Department of Animal Sciences, Purdue University
Ji-Su KIM
National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology
Dong Chul BAEK
National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology
Faculty of Biotechnology, Cheju National University
Jae-Woong LEE
National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology
Sang-Rae LEE
National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology
Myeong-Su KIM
National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology
Sang-Hyun KIM
National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology
Chan-Shick KIM
Faculty of Biotechnology, Cheju National University
Deog-Bon KOO
Department of Biotechnology, Daegu University
Han-Seok KANG
College of Natural Resources and Life Science, Pusan National University
Zae-Young RYOO
School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University
Kyu-Tae CHANG
National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology

Keywords: A disintegrin and metalloprotease (ADAM), Disintegrin domain, Porcine, Sperm-egg interaction

JOURNAL FREE ACCESS

2009 Volume 55 Issue 2 Pages 156-162

DOI https://doi.org/10.1262/jrd.20134

View "Advance Publication" version (December 24, 2008).

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Abstract

In the mouse, ADAM3, a well-characterized testis-specific protein of the A disintegrin and metalloprotease (ADAM) family, has a crucial role in fertilization by mediating sperm binding to the egg zona pellucida. However, little is known about ADAM3 in other species, such as domestic pigs. We have identified porcine ADAM3 and analyzed the protein. RT-PCR and trypsinization of sperm surface proteins revealed that porcine ADAM3 is expressed at high levels in the testis and on the sperm surface. Furthermore, an IVF inhibition assay with a recombinant porcine ADAM3 disintegrin domain showed that treatment of the disintegrin domain effectively prevented pig sperm-egg interactions. In the present study, we demonstrated the presence of ADAM3a and ADAM3b molecules in the pig and examined their roles in fertilization.

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