Data were collected from 60 dairy cows affected with postpartum astasia and analyzed by quantificotion model No.3 to obtain rather reliable bases of rapid diagnosis and emergency treatment of this disorder.
As a result, it was possible to classify the cows into five groups on the basis of seven general clinical signs most commonly encountered. These signs were nutrition, cutaneous temperature, appetite, movement of the rumen, force of the tail, conjunctiva of the eye, and consciousness. Then the concentrations of calcium, magnesium, and phosphorus in serum were estimated in the cows of each group. As a result, it was clarified that the injection with a calcium preparation which had been carried out indiscriminately in dairy cows suffering from postpartum astasia for the purpose of diagnostic treatment could be reduced in number of times without reducing the effect of the treatment.

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Organic information obtained from blood is generally more useful for liver function tests than any other information. At present, many items of these tests can be estimated by using particular kits or micro methods. There is, however, a wide range of the normal value of each of them in animals on account of feeding environment, age, and pregnance. Therefore, it is difficult to make the diagnosis of a given disease on the basis of a small change in value of a given item of the test.
The serum colloidal reaction can detect changes in protein metabolism in the liver sensibly. It does not require any complicated instrument, but clarifies the condition of liver function by estimating several items of the test in a short time. To evaluate this reaction in a more objective manner, 57 serum samples were subjected to such four types of the reaction, ZTT, TTT, Gross' reaction, and Lugol's reaction, as had been performed rather commonly. They were also subjected to biochemical tests to determine the following 12 items: total protein, albumin, inorganic phosphete, total cholesterol, glucose, blood urea nitrogen, uric acid, creatinine, Al-P, GOT, GPT, and LDH. Moreover, the protein fractionation was performed on them by electrophoresis. Then, statistical analysis was made to elucidate to what extent the serum colloidal reaction reflected the condition of the liver. At the same time comparison was made between macroscopical autopsy findings of the liver and the results of the serum colloidal reaction.
As a result, the four types of this reaction, except TTT, showed a high correlation with the r-globulin level and such hepatic changes as atrophy, hypertrophy, and cholangitis. In conclusion, the usefulness of the serum colloidal reaction was demonstrated from the results of analysis by the statistical method.

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It was previously reported thet fatty deposition in hepatocytes could be diagnosed, at such accuracy as applicable in the field, on the basis of quantitative data obtained from general and simplified laboratory tests by the application of the method of quantificotion model No.2. In the present experiment, discriminant analysis was applied to the quantitative data obtained from the biochemical blood test. Its results were used to judge the severity of fatty deposition in hepatocytes. A total of 196 dairy cows were suspected clinically to be affected with hepatic dysfunction. They were examined by the biochemical blood test and divided into three groups on the basis of severity of fatty deposition in hepatocytes; that is, (-), (+- _??_), and ( _??_) . In the discrimination of these groups, there was a fluctuation in the accuracy of discrimination in each group. This accuracy was generally low in all the groups. Nevertheless, when two pairs of two groups of discrimination were combined, each group could be discriminated from any other group at a high accuracy. Therefore, it was suggested that this method of discrimination might be available for the diagnosis of hepatic function in the field.

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Analysis was made on sick cows in which so poor prognosis had been made as to require emergency slaughter from the results of the blood test for screening. The deta used for it were collected from 14 items of the blood test on 25 healthy cows and 24 sick cows in which so poor prognosis had been made as to require emergency slaughter. The methods used were cluster analysis, principal component analysis, discriminast analysis, and analysis of variance.
The results of analysis indicated that prognosis was poor when Ca, Eo, iP, and Mg were low in level and Fib, Glu, GOT, LDH, and T.Bil high in level. It was suggested that it might be necessary for sick cows to be subjected to emergency slaughter when Fib exceeded 1, 200mg/dl, Glu 100mg/dl, GOT 200 KU, LDH 500 WIU, and T.Bil 0.8mg/dl in the cows.

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Analysis was made on factors in the process of manufacture of three kinds of western-style cake, chou á la créme, cream puff, and Mont Blanc. Its purpose was to estimate the degree of influence of each factor on the sanitation quality (the count of coliform bacteria) of the kinds of cake.
The count of coliform bacteria was obtained from each kind of cake by the stamp method, which can be performed readily in the factory. It was used as a criterion. To it were used 11 items of 4 groups and 88 categories as independent variables. The 4 groups and 11 items (listed in parentheses) were as follows : group, No.l, of workers (degree of skillfulness, manner of working, and cleanliness of clothing), group, No.2, of working environment (degree of orderly arrangemest of instruments and utensils, temperature and humidity, and dryness of the floor) . group, No.3, of instruments and utensils (degree of cleanliness of towels, disinfection or not of vessels, and degree of cleanliness of the squeezer) . group, No, 4 of manufacturing process (degree of disinfection of chow á la créme and degree of cooling of this cream) . The 88 categories consisted of 29 of chou á la créme, 31 of cream puff, and 28 of Mont Blanc. Analysis was carried out by the quantification models, Nos.2 and 3, to estimate the significance of these factors and categories. The results oboained are summarized as follows.
The sanitation quality of the three kinds of cake seemed to have been influenced relatively strongly by the degree of skillfulness of workers (group No.l), that of cream puff and Mont Blanc by temperature and humidity, and dryness of the floor (group No.2), that of chou á la créme and cream puff by the degree of cleanliness of towels and that of cream puff and Mont Blanc by the degree of cleanliness of the squeezer (group No.3), and that of chou á la créme by the degree of disinfection (group No.4) .
In brief, the sanitation quality of the three kinds of cake seemed to have been influenced relatively strongly by some items of groups 1 and 3. To improve it in future, it was considered to be necessary to observe the “Standards for Treatment of Dish-Towels and Squeezers” and the “Training and Guidance of Workers” strictly.

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