Production of 3-acetylnivalenol by transgenic Fusarium graminearum expressing Tri13 of type A trichothecene-producer: participation of the encoded cytochrome P450 monooxygenase in type B trichothecene biosynthesis

Kazuyuki MAEDA; Takeshi TOKAI; Hinayo ICHIKAWA; Naoko TAKAHASHI-ANDO; Nobuo OGURA; Katsuyoshi YONEYAMA; Minoru YOSHIDA; Makoto KIMURA

doi:10.2520/myco.62.83

Regular Papers

Production of 3-acetylnivalenol by transgenic Fusarium graminearum expressing Tri13 of type A trichothecene-producer: participation of the encoded cytochrome P450 monooxygenase in type B trichothecene biosynthesis

Kazuyuki MAEDA, Takeshi TOKAI, Hinayo ICHIKAWA, Naoko TAKAHASHI-ANDO, Nobuo OGURA, Katsuyoshi YONEYAMA, Minoru YOSHIDA, Makoto KIMURA

Author information

Kazuyuki MAEDA
Chemical Genomics Research Group, Advanced Science Institute (ASI), RIKEN
Department of Agriculture, Graduate School of Agriculture, Meiji University
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
Takeshi TOKAI
Chemical Genomics Research Group, Advanced Science Institute (ASI), RIKEN
Hinayo ICHIKAWA
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
Naoko TAKAHASHI-ANDO
Department of Applied Chemistry, Faculty of Science and Engineering, Toyo University
Nobuo OGURA
Department of Agriculture, Graduate School of Agriculture, Meiji University
Katsuyoshi YONEYAMA
Department of Agriculture, Graduate School of Agriculture, Meiji University
Minoru YOSHIDA
Chemical Genomics Research Group, Advanced Science Institute (ASI), RIKEN
Makoto KIMURA Corresponding author
Chemical Genomics Research Group, Advanced Science Institute (ASI), RIKEN
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University

Keywords: C-4 hydroxylation, C-15 deacetylation, Fusarium graminearum, Fusarium sporotrichioides, Tri8, type B trichothecenes

JOURNAL FREE ACCESS

2012 Volume 62 Issue 2 Pages 83-90

DOI https://doi.org/10.2520/myco.62.83

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Abstract

Tri13 encodes a cytochrome P450 monooxygenase that catalyzes C-4 hydroxylation of some intermediates in the biosynthesis of type A and type B trichothecenes. This work demonstrated that Tri13 (FsTri13) of Fusarium sporotrichioides, a typical type A trichothecene producer, can participate in the biosynthesis of type B trichothecenes. When FsTri13 was expressed in Fusarium graminearum that produces 3-acetyldeoxynivalenol (3-ADON) as an end product, 3-acetylnivalenol (3-ANIV) was newly detected in the liquid medium. Although 3-ANIV can easily be prepared enzymatically from nivalenol (NIV) by using recombinant trichothecene 3-O-acetyltransferase (TRI101), there has been no conclusive report on the production of 3-ANIV in cultures of Fusarium strains. Possible biosynthetic pathway for production of 3-ANIV was suggested with regard to the function of Tri8, which is responsible for deacetylation of the side-chain O-acetyls in 3-ADON biosynthesis.

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