抄録
A cell recovery method, cells are exposed to chemicals and then cultured in chemical-free medium for a specific period, and toxicity is evaluated based on the recovery level of the cells. Cells exposed to strongly toxic chemicals do no not recover, and the cytotoxicity of some chemicals may be enhanced by chemical-free medium. In this study, to investigate the application of this method for ES-D3 cell differentiation, in addition to cell proliferation, we evaluated 4 types of monomer used for dental composite resin and adhesive, and observed that cells exposed to Bis-GMA did not recover compared to those exposed to the other monomers. The cell differentiation levels were the same as those observed under the conventional conditions, showing that further improvement of the cell recovery test method is necessary.
