Alternatives to Animal Testing and Experimentation
Online ISSN : 2185-4726
Print ISSN : 1344-0411
ISSN-L : 1344-0411
Original Articles
Preparation of Multiple Cell Types as Research Resources from Surplus Tissues Derived from Surgery for Polydactyly
Nozomi SugiharaMotonobu SatohTakuo KosakaAya Kasamatsu-onishiAkifumi MatsuyamaShin EnosawaMakoto HikosakaTsuyoshi KanekoArihiro Kohara
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2017 Volume 22 Issue 2 Pages 155-166


We prepared multiple cell types from surplus finger tissues obtained from surgeries for polydactyly performed in Japanese patients. We tested their suitability as new bioresources for the National Institute of Biomedical Innovation, Health and Nutrition/Japanese Collection of Research Bioresources tissue bank. Dermal fibroblasts, epidermal keratinocytes, melanocytes, preadipocytes, and bone-derived cells were isolated from fresh or frozen tissues and subjected to quality control tests that assessed viability after frozen storage, morphology, microbial contamination, and cell-specific function. We confirmed that the cells exhibited the expected qualities and represented suitable research resources derived from Japanese individuals. Our public human tissue bank will provide these cells to academics and industries as useful alternatives to animal experiments in the research and development of drugs and cosmetics.

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© 2017 Japanese Society for Alternative to Animal Experiments
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