2024 Volume 29 Issue 2 Pages 45-53
Toxicity evaluation methods for nanomaterials often involve animal experiments and in vitro studies using cells and bacteria. Various metal nanoparticles, including platinum, silver, and zinc, are used in the medical field. Platinum nanoparticles can decompose superoxide radicals (O₂⁻) within cells, showing superoxide dismutase (SOD)-like activity. This study examined the effects of equal-volume mixtures of platinum and silver nanoparticles, and platinum and zinc nanoparticles, on SOD-like activity, oxidative stress, and cell viability compared to individual silver and zinc nanoparticles. Results indicated that the SOD-like activity was highest (97.61%) with the platinum-silver mixture, while it was lower with the platinum-zinc mixture. Oxidative stress results showed no significant differences, and cell viability under mixed conditions was intermediate between the single metals with no specific increase or decrease. The study suggests that a platinum-silver mixture exhibits higher SOD-like activity and slightly suppresses intracellular ROS generation compared to individual metals. Further research is needed to determine the relationship between ROS, aging, and carcinogenesis using alternative methods in animal experiments. Additionally, elucidating the functions and applications of metal nanomaterials under various mixing conditions is important for future applications.
