日本ダニ学会誌
Online ISSN : 1880-2273
Print ISSN : 0918-1067
ISSN-L : 0918-1067
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Effectiveness of essential oils of medicinal plants at reducing the amounts of allergen produced by the European house dust mite, Dermatophagoides pteronyssinus (Trouessart)
Ammorn INSUNGJarongsak PUMNUANVanna MAHAKITTIKUNTeerapong WANGAPAI
著者情報
キーワード: ELISA, spent mite medium, direct spray
ジャーナル フリー

2016 年 25 巻 Supplement1 号 p. S179-S184

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Essential oils obtained from three medicinal plant species, namely, cinnamon (Cinnamomum bejolghota [Buch.-Ham.] Sweet), citronella grass (Cymbopogon nardus Rendle), and clove (Syzygium aromaticum [L.] Merr. & L.M. Perry), were sprayed on allergenic materials (house dust plus spent mite medium produced by the European house dust mite, Dermatophagoides pteronyssinus [Trouessart]) to evaluate the effects of the oils on allergen levels. A 1% solution of each essential oil in 95% ethanol was sprayed on the allergenic materials (which contained about 50μg of allergen per gram of material), and 95% ethanol, water, and untreated allergenic materials were used as controls. The sprayed materials were allowed to stand for 48h, and then allergen levels were analyzed by means of an enzyme-linked immunosorbent assay method. The results showed that the essential oil from C. bejolghota reduced the amount of allergen>99.4%, and the essential oils from C. nardus and S. aromaticum reduced the amounts by 77.1% and 74.0%, respectively. In contrast, the 95% ethanol control reduced the amount of allergen by only 9.8%.
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© 2016 by The Acarological Society of Japan
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