Histological and Histochemical Studies on the Male Accessory Genital Glands and the Derivations of the Spermatophore Components from the Accessory Glands in Haemaphysalis longicornis(Acari: Ixodidae)

Tomohide MATSUO; Takayuki MORI; Satoshi SHIRAISHI

doi:10.2300/acari.6.1

Tomohide MATSUO, Takayuki MORI, Satoshi SHIRAISHI

Author information

Keywords: spermatophore, male accessory glands, copulation, Haemaphysalis longicornis, sperm transfer, tick

JOURNAL FREE ACCESS

1997 Volume 6 Issue 1 Pages 1-10

DOI https://doi.org/10.2300/acari.6.1

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Published: May 25, 1997 Received: September 17, 1996 Available on J-STAGE: June 28, 2010 Accepted: October 23, 1996 Advance online publication: - Revised: -
Correction information
Date of correction: June 28, 2010 Reason for correction: - Correction: TITLE Details: [in Japanese]

Date of correction: June 28, 2010 Reason for correction: - Correction: ABSTRACT Details: [in Japanese]

Date of correction: June 28, 2010 Reason for correction: - Correction: AUTHOR Details: Wrong : Tomohide MATSUO¹⁾, Takayuki MORI²⁾, Satoshi SHIRAISHI²⁾
Right : Tomohide MATSUO¹⁾, Takayuki MORI¹⁾, Satoshi SHIRAISHI¹⁾

Date of correction: June 28, 2010 Reason for correction: - Correction: AFFILIATION Details: Wrong :
1) Zoological Laboratory, Faculty of Agriculture,
2) Kyushu University,

Right :
1) Zoological Laboratory, Faculty of Agriculture, Kyushu University

Date of correction: June 28, 2010 Reason for correction: - Correction: KEYWORD Details: Wrong : Key words: spermatophore, male accessory glands, copulation, Haemaphysalis longicornis, sperm transfer,tick
Right : spermatophore, male accessory glands, copulation, Haemaphysalis longicornis, sperm transfer, tick

Date of correction: June 28, 2010 Reason for correction: - Correction: CITATION Details: Wrong : E
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Right : Balashov, Y. S. (1972): Bloodsucking ticks(Ixodoidea)-Vectors of diseases of man and animals. Translated by O. G. Strekalovski, Misc. Publs. Entomol. Soc. Am., 8: 161-376.
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El Shoura, S. M.(1987): Fine structure of the vasa deferentia, seminal vesicle, ejaculatory duct, and accessory gland of male Ornithodoyos(Pavlovskyella)erraticus(Acari: Ixodoidea: Argasidae). J. Med. Entomol., 24: 235-242.
Feldman-Muhsam, B.(1967a): Spermatophore formation and sperm transfer in Ornitodoros ticks. Science, 156: 1252-1253.
Feldman-Muhsam, B.(1967b): The components of the spermatophore, their formation and their biological functions in argasid ticks. Proc. 2nd Int. Cong. Acarology, 357-362.
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Feldman-Muhsam, B. and S. Borut(1983): On the spermatophore of ixodid ticks. J. Insect Physiol., 29: 449-457.
Feldman-Muhsam, B. and S. Borut(1984): Differences in the structure of the spermatophore between argasid and ixodid ticks. In: Acarology VI, Vol. 1(eds., Griffiths, D. A. and Browman, C. E. ), pp. 504-511. Ellis Horwood Limited, Chichester.
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Abstract

Matsuo, T., T. Mori and S. Shiraishi, 1997. Histological and histochemical studies on the male accessory genital glands and the derivations of the spermatophore components from the accessory glands in Haemaphysalis longicornis(Acari: Ixodidae). J. Acarol. Soc. Jpn., 6(1): 1-10.
The external shape and histological changes of the male accessory genital glands during feeding and after copulation were observed in Haemaphysalis longicornis. Histochemical properties between secretory granules of the accessory glands and components of the spermatophore were also compared. The accessory glands consisted of two single and five paired lobes opening into the collecting duct(CD)lying on the floor of the body cavity.The single dorso-median lobe(DML)opened to the posterior end of the CD and extended in an antero-dorsal direction. The single medio-ventral lobe(MVL)opened to the antero-ventral region of the CD. Five paired lobes were on both sides of the CD;the antero-ventral lobe(AVL), the latero-ventral lobe(LVL), the dorsolateral lobe(DLL), the postero-lateral lobe(PLL)and the postero-ventral lobe(PVL)from anterior to posterior. Epithelia of the lobes showed remarkable histological changes synchronized with feeding and copulation: Secretory granules contained in the epithelia increased in number during feeding and were almost completely released after copulation. These histological changes suggest that the secretions of the accessory glands form the spermatophore. The histochemical comparisons showed that the outer and inner layers of the ectospermatophore were, respectively, derived from the AVL and the DLL(PLL), and three types of granules in the spermatophore were transferred from the WVL and the LVL.

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