Abstract
A novel tissue consisting of carboxymethylated chitosan (CM-Chitosan) with various degree of substitution of carboxymethylation/degree of deacethylation (DS/DD=COOH/NH2) was developed. In this adhesive, CAD works as a crosslinking reagent of amino groups of CM-Chitosan. The boding time of CAD/CM-Chitosan adhesive reached equilibrium within 5 min when DS/DD of CM-Chitosan was 0.27 and 0.76. While, the bonding time of the CM-Chitosan whose DS/DD was 0.52 rapidly increased within 5 min, however, it took 30 min to become constant level. The bonding strength decreased with increasing CAD concentration when DS/DD of CM-Chitosan was 0.27 and 0.76. On the other hand, bonding strength increased up to CAD concentration of 5 mM and then decreased when DS/DD of CM-Chitosan was 0.52. It was clarified that the resulting bonding strength of CM-Chitosan (DS/DD=0.52) was 4-fold higher than that of fibrin-based adhesive which is commonly used in clinical field.
