Appearance of Iridescence in the Tissues of the Rice Stem Borer Larvae, Chilo suppressalis WALKER, Infected with Chilo Iridescent Virus : (Lepidoptera : Pyralidae)
1966 年 1 巻 3 号 p. 130-137
詳細
1966 年 1 巻 3 号 p. 130-137
Infection of various tissues of Chilo suppressalis larvae with Chilo oiridescent virus (CIV) was examined by the appearance of iridescence. The CIV infected larvae were obtained by intrahemocoelic inoculation of CIV suspension under aseptic condition. The inoculated larvae ususally began to turn purplish 5 days after inoculation at 25°C. Pericardial cells and suboesophageal gland cells showed iridescence at first the iridescence was most prominent. The iridescence could be seen in these cells as early as 3 days following inoculation. Fat bodies showed the next prominent iridescence. Fat body lobules underneath the epidermis showed iridescence 3 days after inoculation, but large lobes distributed around alimentary canal showed iridescence much later and the iridescence was not prominent. Silk glands and salivary glands showed iridescence on some parts of the glands at 4 days following inoculation, but the iridescence appeared only on the surface of the gland. Hemocytes began to show iridescence 5 days after inoculation. In the larvae of advanced infection, hemolymph was turbid. Epidermal cells showed prominent iridescence 6 days after inoculation. Alimentary canals and Malpighian tubules began to show iridescence at 5 days following inoculation, but the iridescence seemed to appear only on the surface of them. In the prothoracic glands, iridescence appeared only in the posterior regious of the glands. At an advanced stage of infection, tracheal epithelium showed iridescence. Nervous system, gonads, and muscles did not show iridescence.
