Applied Entomology and Zoology
Online ISSN : 1347-605X
Print ISSN : 0003-6862
ISSN-L : 0003-6862
Detection of Specific Antigen of the Infectious Flacherie Virus of the Silkworm, Bombyx mori L. by Electrosyneresis
Yasutaka SEKIJIMAAkira OHTSU
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1976 年 11 巻 4 号 p. 356-362

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Electrosyneresis (ES) method was successfully applied to the detection of the specific antigen on infectious flacherie virus of the silkworm (IFV-specific antigen) with the following results.Precipitated line was formed by ES when the IFV-specific antigen was placed on the cathode side and anti-IFV serum was placed on the anode side. The ES method was far better than the micro-Ouchterlony method in the sensitivity, and the precipitated line was observed at 32-fold dillution of IFV-specific antigen. The IFV-specific antigen was detected from the midgut of silkworms which had been administered with heavy and light inocula of the IFV. This was found 4-5 days earlier than the appearance of the symptom. The ES method is practically applicable to the rapid diagnosis of infectious flacherie of the silkworm.
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© the Japanese Society of Applied Entomology and Zoology
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