Abstract
The localization studies on alkaline and acid phosphataes, in alimentary tract, salivary glands and malpighian tubules of both the just hatched nymphs and adults Periplaneta americana were made at pH 9.2 and 5.2 respectively using sodium β-glycerophosphate as substrate. The alkaline phosphatase was found to be widely distributed in alimentary tract, salivary glands and malpighian tubules of both the nymphs and adults. The acid phosphatase activity was completely lacking in the just hatched nymphs, whereas in the adults the positive activity was completely shown in midgut, salivary glands and malpighian tubules. The functional significance of alkaline phosphatease was discussed on the basis of its presence at the sites of active solute trasporation, chitinization, growth and degeneration. Importance of acid phosphatase is not well understood. Perhaps it plays some role in digestion.