The mode of action of insert hormone and its related substance upon the metabolism of diapausing pupa was studied on the pupa of Samia cynthia pryeri. The brain was extirpated from each pupa at the dormant stage. The brainless pupae were kept at 25°C for 5 weeks prior to use.
14C-glucose of 0.1 μc was injected with each of the following substances, such as ecdysone, proteinic brain hormone or cholesterol having brain hormone activity, into the pupae respectively. Twenty-four hours following injection, the incorporation of radioactive glucose into carbon dioxide, trehalose, glycogen, crude lipid, and crude protein was measured by several methods, obtaining the following results.All of the substances injected acted on carbohydrate metabolism in the brainless pupa and they promoted the biosynthesis of trehalose. In control insect without injection of hormones or cholesterol, however, radioactive glucose was incorporated into glycogen in the fat body and scarcely incorporated into blood trehalose. On the other hand, incorporation of
14C-glucose to crude lipid and crude protein showed a low value, though either insect hormone or cholesterol was injected. From the afore-going results, it is concluded that insect hormones, ecdysone and brain hormone, are conerned with carbohydrate metabolism of Samia cynthia pryeri.
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