1990 Volume 25 Issue 4 Pages 467-473
Sex pheromone of the three-spotted plusia, Acanthoplusia agnata, was extracted from abdominal tips of virgin females. The crude extract was purified by Florisil column chromatography, HPLC, and gas chromatography. Male moths were released and captured in a field cage to detect the activity of fractionated samples. The sex pheromone components of A. agnata were estimated to be (Z)-7-and (Z)-9-dodecenyl acetates according to analysis by capillary GC and GC-MS. A plastic, capsule-loaded 1.1 mg of mixture of the 2 compounds in a 10 : 1 ratio attracted male moths under field conditions. From these data, the sex pheromone of A. agnata was determined to be a 10 : 1 mixture of (Z)-7-and (Z)-9-dodecenyl acetates. This mixture also attracted male moths of Chrysodeixis eriosoma.