Tissue Culture of the Rice Stem Borer, Chilo suppressalis WALKER (Lepidoptera : Pyralidae) : III. Effects of Temperatures and Cold-Storage on the Multiplication of the Cell Line from Larval Hemocytes
1968 年 3 巻 1 号 p. 1-4
詳細
1968 年 3 巻 1 号 p. 1-4
Multiplication of cells in a cell line from Chilo suppressalis hemocytes was studied at various temperatures and after cold-storage for various periods. The maximum cell multiplication was obtained at 25°C. The cells increased in number at temperatures ranging from 22°C to 28°C, but they decreased in number at 20°C and 30°C. The cells recovered their viability after cold-storage at 5°C for 30, 60, 90 and 120 days. The degree of cell multiplication in the cold-storaged cells was slightly inferior to the controls. The Chilo iridescent virus carrier state was maintained in all the experimental groups.
