Changes in Zymograms of Some Dehydrogenases and Hydrolases in Several Organs of the Silkworm, Bombyx mori L. (Lepidoptera : Bombycidae) during Metamorphosis

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Electrophoretic separation in agar gel on various enzymes of silkworm tissues was carried out. The intestine had generally intense bands of various enzymes moving toward the anode. Fat body, integument and ovary showed active bands of some enzymes such as malate dehydrogenase and glucose-6-phosphate dehydrogenase. However, clear band could not be detected in the blood. Malate, α-glycerophosphate, and lactate dehydrogenases, leucine aminopeptidase, proteinase and alkaline phosphatase occurred in multiple forms, whereas only one positively migrating band was detected in silkworm tissues for isocitrate and glucose-6-phosphate dehydrogenases. Regarding zymograms of extracts from intestine, the staining intensity of dehydrogenating enzymes generally decreased with the progression of development. On the other hand, in zymograms of hydrolytic enzymes the high staining intensity at the last larval stage fell at the prepupal and early pupal stage and rose again at the middle pupal stage.