A Continuous Cell Line from Pupal Ovaries of the Common Cutworm, Spodoptera litura (Lepidoptera: Noctuidae)

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A continuously growing cell line was obtained from the culture of the pupal ovaries of the common cutworm, Spodoptera litura. The primary culture was initiated in MGM-450 medium fortified with 5% fetal bovine serum (FBS) and 3% Antheraea pernyi hemolymph heated at 60°C for 30 min. However, the cell line could grow in the MGM-450 medium with 10% FBS or in the MM medium with 3% FBS. The population doubling time was about 43 h in either medium at 25°C. The cells could be stored in the culture medium containing 10% glycerol at -100°C. The karyotype was typical to lepidopteran cell lines. The cell line was susceptible to S. litura nuclear polyhedrosis virus. The cell line could be distinguished from other cell lines derived from other insect species by comparison of the isozyme patterns of isocitrate dehydrogenase, malic enzyme, phosphoglucose isomerase and phosphoglucomutase. The cell line was designated as TUAT-SpLi-221.