Isolation and characterization of a strain of Bacillus thuringiensis serovar kenyae encoding olny δ-endotoxin Cry1E

抄録

Bacillus thuringiensis STB-3 which was isolated from a Korean soil sample showed high toxicity to Spodoptera exigua. Its crystal toxin was bipyramidal-shaped with a molecular weight of 130 kDa. The H-serotype of STB-3 was identical to B. thuringiensis serovar kenaye (H4a4c). However, PCR analysis using Spodoptera-specific cry gene primers showed that STB-3 had only the cry1E crystal gene, differing from that of serovar kenyae (reference strain) which contains cry1Ab, cry1Ac and cry1E crystal genes.