Abstract
1) No enzyme activities for succinic dehydrogenase and DPN-diaphorase in myelin sheath of normal, degenerating and regenerating sciatic nerve could be demonstrated by histochemical methods. Non-specific esterase activity could be found in the nerve fiber but not in axons, their distribution in nerve fiber was in irregular pattern with fine positive granules.
2) The myelin structure in fresh frozen sections showed circular thin threads, and this structure coincided with electron microscopic view.
3) Alteration of succinic dehydrogenase and DPN-diaphorase in the proximal stump following sciatic neurotomy: Axonal swelling accompanied with a damming of succinic dehydrogenase and DPN-diaphorase at the end site of axon and increased enzyme activity in the Schwann cell were noted.
4) The changes in monoamine oxidase activity in the regenerating nerve fiber in proximal stump showed a very interesting localization, during the regeneration of sciatic nerve, the mid-portion of proximal stump demonstrated strong monoamine oxidase activity in the outer surface of the axon and this may indicate the axon-Schwann cell membrane.
