Analysis of Charged Cyclodextrin Derivatives by On-Line Capillary Electrophoresis-Ionspray-Mass Spectrometry

Abstract

On-line capillary electrophoresis-ionspray-mass spectrometry (CE-ISP-MS) was applied to a composition analysis of charged cyclodextrin (CD) derivatives. Quaternary ammonium β-CD (QA-β-CD) and carboxymethylated γ-CD (CM-γ-CD), which were used as chiral selectors for enantiomer separations by CE, were analyzed. As for QA-β-CD, eight components having different degrees of the quaternary ammonium substitution could be identified based on their mass spectra. The components were detected as either molecular ions or fragment ions. On the other hand, six components of CM-γ-CD were detected as bivalent molecular ions. Such composition analyses were difficult using CE with only the UV detector. CE-MS was found to be a promising technique for a composition analysis of mixed compounds.