Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Prediction of Measurement Precision by Chemometric Invalidation of Flow Pulsation in Refractive Index Detection of Liquid Chromatography
Chikako YOMOTAYoko TAGASHIRASatoshi OKADAYuzuru HAYASHIRieko MATSUDA
Author information
JOURNAL FREE ACCESS

1999 Volume 15 Issue 6 Pages 549-554

Details
Abstract
The standard deviation and relative standard deviation of measurements in the refractive index detection of liquid chromatography are predicted based on the 1/f fluctuation model which is made up of white noise and the Markov process. First, repeated measurements and Monte Carlo simulation corroborate the reliability of this prediction in the absence of flow pulsation. Pulsation, if any, can be distinguished in the frequency space from the 1/f noise which is common to most analytical instruments. In this case, the uncertainty prediction is also available without modifying the prediction theory, if a zero window is set at the time period of a fundamental tone of the pulsation (virtual invalidation of pulsation). The zero window is used for determining the zero level relative to which the signal intensities are summed for a signal area (measurement). A maltose determination is taken as an example.
Content from these authors
© 1999 by The Japan Society for Analytical Chemistry
Previous article Next article
feedback
Top