Peroxidase-Like Activity of Ion Exchange Resins Modified with Metal-Porphine in Fluorescent Flow Injection Analysis

Abstract

Peroxidase-like activity of anion-exchange resins modified with metal-tetrakis(sulfophenyl)porphine (M-TSPP_r, M=Fe³⁺, Co³⁺ and Mn³⁺) were evaluated in the flow injection analysis of hydrogen peroxide using a fluorescent dimmer-formation reaction from chromogens, such as tyramine. A column (2.1 mm×50 mm) packed with Fe³⁺-TSPP_r and a tube (0.8 mm×50 mm) packed with Mn³⁺-TSPP_r accelerated the dimmer-formation reaction, indicating that they exhibit peroxidase-like activity just like the peroxidase-immobilized-column in the flow injection system.