Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Production and Characterization of Monoclonal Antibodies Recognizing the Asymmetric Center of Optically Active Bufuralol and Its Metabolites
Shigeo IKEGAWANi Made Ria ISRIYANTHINorihiro KOBAYASHIShinichi MIYAIRIJunichi GOTO
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2000 Volume 16 Issue 1 Pages 31-35

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Abstract

A specific antibody has been produced for developing immunoaffinity chromatography of optically active bufuralol and its 1′-oxidized metabolites. Spleen cells from a BALB/c mouse, which had been immunized with the (1R)- or (1S)-1′-oxobufuralol O-carboxymethyl oxime-bovine serum albumin conjugate, were fused with P3/NS1/1-Ag4-1 myeloma cells. After screening by enzyme-linked immunosorbent assay (ELISA) using a β-galactosidase-labeled antigen, one for (1R)-bufuralol and three kinds for (1S)-bufuralol of antibody-secreting hybridoma clones were established. The monoclonal antibodies, Ab-R-12 and Ab-S-1,were determined to have favorable characteristics for preparing the immunosorbent, because each of them was specific for (1R)- or (1S)-bufuralols and its corresponding 1′-oxidized metabolites but showed significantly low cross-reactivities with their antipodes including 1′-oxidized metabolites.

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© 2000 by The Japan Society for Analytical Chemistry
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