Abstract
This study examined enantiomeric separation by capillary electrochromatography with charged polyacrylamide gels incorporating chiral selectors, in which electroosmotic flow would be generated. The columns are capillaries filled with a negatively or positively charged polyacrylamide gel, a so-called monolithic stationary phase, incorporating polymeric β-cyclodextrins as chiral selectors. The capillary wall is activated first with a bifunctional reagent to make the resulting gel bind covalently to the inner surface of the fused-silica tubing. Enantiomeric separations of two cationic and a neutral compounds were achieved using negatively charged polyacrylamide gel-filled capillaries incorporating poly β-cyclodextrin and carboxymethyl β-cyclodextrin polymer and a mobile phase of 200 mmol dm-3 Tris-300 mmol dm-3 boric acid buffer (pH 9.0). Enantiomeric separations of twelve anionic compounds were also achieved using positively charged polyacrylamide gel-filled capillaries incorporating poly β-cyclodextrin and a mobile phase of 200 mmol dm-3 Tris-300 mmol dm-3 boric acid buffer (pH 8.1). High efficiencies of up to 240000 plates m-1 were obtained. The within-run reproducibilities of retention time and separation factor were good.
