Abstract
A protein assay with the determination sensitivity at nanogram levels has been developed based on measurement of enhanced resonance light scattering (RLS). In a neutral aqueous medium, the interactions of ABGX with different proteins were found to result in strongly enhanced RLS signals. With the enhanced RLS signals at 398.0 nm, proteins, including bovine serum albumin (BSA), human serum albumin (HAS), pepsin (Pep) and cellulase (Cel), can be determined with the limit of detection below 30 ng/ml. The results of determinations for artificial samples were in agreement with the desired values, and those for human serum samples were identical with those obtained according to the Bradford method using CBB G-250.
