Peroxidase-Like Catalytic Activity of Metal Complexes of Thiacalix[4]arenetetrasulfonate on the Modified Ion-Exchanger and Its Application to the Determination of Hydrogen Peroxide¹

Abstract

The peroxidase-like catalytic activity of ion-exchangers modified with some metal complexes of thiacalix[4]arenetetrasulfonate (Me-TCAS, Me=Fe³⁺, Fe²⁺, Co²⁺, Mn²⁺, Cu²⁺, Zn²⁺ and Ni²⁺) was investigated based on a color reaction catalyzed by peroxidase. The ion-exchanger modified with Fe³⁺-TCAS showed the highest activity among the metal complexes tested, and was applied to the determination of hydrogen peroxide. The calibration curve for the ion-exchanger modified with Fe³⁺-TCAS was linear over the range from 10 to 100 μg of hydrogen peroxide in a 1 ml sample solution. Moreover, the method using glucose oxidase and the ion-exchanger modified with Fe³⁺-TCAS was applied for the determination of glucose. The ion-exchanger modified with Fe³⁺-TCAS may be applicable for the determination of hydrogen peroxide in place of peroxidase.