Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Photo-responsive Retention Behavior of Azobenzene-modified Cyclodextrin Stationary Phase in Micro-HPLC
Tatsuro NAKAGAMAKazuya HIRASAWAKatsumi UCHIYAMAToshiyuki HOBO
Author information
JOURNALS FREE ACCESS

2001 Volume 17 Issue 1 Pages 119-124

Details
Abstract

An azobenzene-modified γ-cyclodextrin stationary phase (Azγ-CDSP) was prepared and its photo- and temperature-responses for the retention of perylene and pentacene were investigated using a mixture of methanol and water as the mobile phase in micro-HPLC. The retention of perylene slightly increased, whereas that of pentacene significantly decreased by UV light irradiation to Azγ-CDSP. These retentions recovered upon irradiation with visible light. Both retentions decreased upon an increase in the column temperature. It was presumed that the trans-azobenzene moiety acts as a preventive cap for perylene and a spacing for pentacene in filling the CD cavity. An azobenzene-modified stationary phase changed its retention behavior with the column temperature and the light irradiation. An improvement in the micro-HPLC system and the optimization of the molecular structure of the photo-responsive stationary phase would provide selective retention control by the irradiation of light in micro-separation systems.

Information related to the author
© 2001 by The Japan Society for Analytical Chemistry
Previous article Next article
feedback
Top