A Novel Two-Enzyme Amperometric Electrode for Lactose Determination

Abstract

Coimmobilization of β-galactosidase and glucose oxidase in a redox polymer, polyvinylferrocenium perchlorate (PVF⁺ClO₄^-), led to the development of an enzyme electrode for the determination of lactose. The amperometric response of the electrode was measured at +0.70 V vs. SCE, which was due to the electrooxidation of enzymatically produced H₂O₂. The effects of the substrate and buffer concentrations as well as the pH on the electrode response were elucidated.