A Novel Piezoelectric Biosensor for the Detection of Phytohormone β-Indole Acetic Acid

Jin LI; Zhao-Yang WU; Lang-Tao XIAO; Guang-Ming ZENG; Guo-He HUANG; Guo-Li SHEN; Ru-Qin YU

doi:10.2116/analsci.18.403

Original Papers

A Novel Piezoelectric Biosensor for the Detection of Phytohormone β-Indole Acetic Acid

Jin LI, Zhao-Yang WU, Lang-Tao XIAO, Guang-Ming ZENG, Guo-He HUANG, Guo-Li SHEN, Ru-Qin YU

Author information

Jin LI
State Key Laboratory of Chemo/Biosensing and Chemometrics, Chemistry and Chemical Engineering College, Department of Environmental Science and Engineering, Hunan University
Zhao-Yang WU
State Key Laboratory of Chemo/Biosensing and Chemometrics, Chemistry and Chemical Engineering College, Department of Environmental Science and Engineering, Hunan University
Lang-Tao XIAO
Key Laboratory of Phytohormones, Hunan Agricultural University
Guang-Ming ZENG
State Key Laboratory of Chemo/Biosensing and Chemometrics, Chemistry and Chemical Engineering College, Department of Environmental Science and Engineering, Hunan University
Guo-He HUANG
State Key Laboratory of Chemo/Biosensing and Chemometrics, Chemistry and Chemical Engineering College, Department of Environmental Science and Engineering, Hunan University
Guo-Li SHEN
State Key Laboratory of Chemo/Biosensing and Chemometrics, Chemistry and Chemical Engineering College, Department of Environmental Science and Engineering, Hunan University
Ru-Qin YU
State Key Laboratory of Chemo/Biosensing and Chemometrics, Chemistry and Chemical Engineering College, Department of Environmental Science and Engineering, Hunan University

JOURNAL FREE ACCESS

2002 Volume 18 Issue 4 Pages 403-407

DOI https://doi.org/10.2116/analsci.18.403

Details

Abstract

A novel piezoelectric immunosensor has been developed for the determination of β-indole acetic acid (IAA) in dilute solutions. The detection is based on competitive immunoreaction between a hapten (IAA) and an antigen (IAA-BSA, hapten-protein conjugation) bound to an anti-IAA antibody, immobilized on a quartz crystal microbalance (QCM). The frequency change (y) of the sensor caused by antigen is linearly related to the logarithm of the concentration of IAA (x) in the range of 0.5 ng/ml - 5 μg/ml with a regression equation of the form y = -23x + 151 (r = 0.9937).

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