Abstract
The electrochemical behavior of thiamine on a self-assembled electrode of L-cysteine (Cys/SAM/Au) has been investigated and Cys/SAM/Au can be used to detect thiamine using square-wave voltammetry (SWV). At pH 11.40 Britton-Robinson buffer, thiamine exhibits a well-defined anodic peak on Cys/SAM/Au. Under the optimized conditions, the anodic peak current of SWV was linear with the content of thiamine in the range of 1.1 × 10-8 - 2.2 × 10-6 mol/L; the detection limit was 5.5 × 10-9 mol/L. The method was successfully applied to the determination of thiamine in pharmaceutical preparations.
