Range of Separation of Potential Tool for Bioseparation, Microchip Electrophoresis System, for DNA Polymorphisms on the Human Y-Chromosome

Mohammad JABASINI; Feng XU; Fuquan DANG; Toshikatsu SHINKA; Yutaka NAKAHORI; Yoshinobu BABA

doi:10.2116/analsci.19.175

Notes

Range of Separation of Potential Tool for Bioseparation, Microchip Electrophoresis System, for DNA Polymorphisms on the Human Y-Chromosome

Mohammad JABASINI, Feng XU, Fuquan DANG, Toshikatsu SHINKA, Yutaka NAKAHORI, Yoshinobu BABA

Author information

Mohammad JABASINI
Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, CREST, Japan Science and Technology Corporation (JST)
Feng XU
Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, CREST, Japan Science and Technology Corporation (JST)
Life Science Department, Shimadzu Corporation
Fuquan DANG
Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, CREST, Japan Science and Technology Corporation (JST)
Toshikatsu SHINKA
Department of Human Genetics Public Health, School of Medicine, The University of Tokushima
Yutaka NAKAHORI
Department of Human Genetics Public Health, School of Medicine, The University of Tokushima
Yoshinobu BABA
Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, CREST, Japan Science and Technology Corporation (JST)
Single-Molecule Bioanalysis Laboratory, National Institute of Advanced Industrial Science and Technology (AIST)

JOURNAL FREE ACCESS

2003 Volume 19 Issue 1 Pages 175-176

DOI https://doi.org/10.2116/analsci.19.175

Details

Abstract

For the requirement of a high, fast and sufficient technology to suit the needs of 21st century biotechnology, the separation range of a microchip electrophoresis system was studied. Two DNA fragments on the human Y-chromosome, SY594 (82 bp) and 12f2 (88 bp), were successfully separated with a reproducibility of 1.9% and an accuracy of 2.8%. Then, a mixture of 10 DNA markers ranging from 61 bp to 189 bp was successfully separated with high resolution. All of these results demonstrate the superiority of microchip electrophoresis as a tool for 21st century bioseparation.

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