Preparation and a Time-Resolved Fluoroimmunoassay Application of New Europium Fluorescent Nanoparticles

Abstract

New silica-based europium fluorescent nanoparticles having surface amino groups were prepared by a covalent bindingcopolymerization technique. In the nanoparticles, the fluorescent Eu³⁺ chelate molecules were covalently bound to silicon atoms to protect the nanoparticles from dye leaking in bio-applications. The amino groups on the surface of nanoparticles made the surface modification and bioconjugation of nanoparticles easier. The nanoparticles were characterized and developed as a new type of fluorescence probe for a highly sensitive time-resolved fluoroimmunoassay (TR-FIA) of human hepatitis B surface antigen (HBsAg).