Capillary Electrophoresis-Chemiluminescence Detection System Equipped with a Consecutive Sample-Injection Device

Abstract

We have developed a consecutive sample-injection device for capillary electrophoresis, which comprises one four-way cock, two syringe pumps, and an interface part taking advantage of two three-way Teflon joints. Sample introduction into the capillary is made hydrodynamically by pressure, caused by the flow of the sample solution at the tip of the capillary inlet. We combined the injection device with a capillary electrophoresis-chemiluminescence detection system. A mixture solution of N-(4-aminobutyl)-N-ethylisoluminol, isoluminol isothiocyanate, and luminol was analyzed as a model sample by the present system. The sample solution was consecutively injected and detected with about a 230 s interval. The present capillary electrophoresis-chemiluminescence detection system with the consecutive sample injection device features easy and rapid operation, an inexpensive apparatus, high sensitivity, as well as consecutive analysis.