Drug Response Assay System in a Microchip Using Human Hepatoma Cells

Yuki TANAKA; Kiichi SATO; Masayuki YAMATO; Teruo OKANO; Takehiko KITAMORI

doi:10.2116/analsci.20.411

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Drug Response Assay System in a Microchip Using Human Hepatoma Cells

Yuki TANAKA, Kiichi SATO, Masayuki YAMATO, Teruo OKANO, Takehiko KITAMORI

Author information

Yuki TANAKA
Department of Applied Chemistry, School of Engineering, The University of Tokyo
Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency
Kiichi SATO
Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Micro Chemistry Group, Special Research Laboratory for Optical Science, Kanagawa Academy of Science and Technology
Masayuki YAMATO
Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University
Teruo OKANO
Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University
Takehiko KITAMORI
Department of Applied Chemistry, School of Engineering, The University of Tokyo
Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency
Micro Chemistry Group, Special Research Laboratory for Optical Science, Kanagawa Academy of Science and Technology

JOURNAL FREE ACCESS

2004 Volume 20 Issue 3 Pages 411-413

DOI https://doi.org/10.2116/analsci.20.411

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Abstract

A microchip-based cell response assay system to an anticancer agent was developed. The hepatoma cell line HepG2 was used to assess the effects of an anticancer agent, doxorubicin. The required cell number was reduced by two orders, and the observation of the time course of cell response became possible. The system clearly showed that treatment with higher doses of the drug or longer exposure times gave more effects to cells. The possibilities of novel drug response studies or toxicity assay system were demonstrated.

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