Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Native Fluorescence Flow-through Optosensor for the Fast Determination of Diphenhydramine in Pharmaceuticals
Isabel PASCUAL REGUERAMaría GUARDIA RUBIOAntonio MOLINA DÍAZ
Author information
JOURNALS FREE ACCESS

2004 Volume 20 Issue 5 Pages 799-803

Details
Abstract

A single, rapid flow-through optosensor spectrofluorometric system is proposed for the determination of diphenhydramine in different pharmaceutical preparations. This sensor was developed in conjunction with a monochannel flow-injection analysis system with fluorometric solid-phase transduction. Diphenhydramine was directly injected into a carrier stream of ethanol/water, 50% (v:v), and transitorily retained on a sorption gel Sephadex G-15 placed in the detection area into the cell. The determination was carried out without any derivatization reaction by directly measuring the intrinsic fluorescence of the analyte and using the peak height as an analytical signal. The chemical and instrumental variables were optimized, and the influence of some foreign substances that can be found in typical pharmaceutical samples containing diphenhydramine was also investigated. Diphenhydramine could be determined in the concentration ranges of 0.5 - 8 µg ml-1 and 0.1 - 1.2 µg ml-1 with detection limits of 0.088 and 0.019 µg ml-1 at sampling rates of 30 and 19 h-1 for 200 and 800 µl of the sample volume, respectively.

Information related to the author
© 2004 by The Japan Society for Analytical Chemistry
Previous article Next article
feedback
Top