Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Spectral Properties of the Association Nanoparticle System of Ciprofloxacin-Phloxine and Its Application to Fluorescence Analysis
Jieming ZOUZhiliang JIANGLisheng WANGTingsheng LIQinye LIU
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JOURNAL FREE ACCESS

2004 Volume 20 Issue 6 Pages 967-970

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Abstract
There is a fluorescence peak at 570 nm, and a maximum absorption peak at 560 nm for phloxine (PHLO) in a pH 7 water solution. Under these conditions, the ciprofloxacin cation (CPFX+) and PHLO- combine into hydrophobic CPFX-PHLO association molecule by means of static gravitation. There are stronger van der Waals forces and hydrophobic forces among the CPFX-PHLO molecules. Thus, they aggregate automatically to the (CPFX-PHLO)n association nanoparticle in red-violet color. That was characterized by scan electron microscopy (SEM), hyperfiltration and dialysis tests. In 0.04 M HCl, the red-violet nanoparticles exhibited a Rayleigh scattering peak at 470 nm, a resonance scattering peak at 580 nm, a maximum absorption wavelength at 565 nm, and a fluorescence peak at 450 nm. The fluorescence analytical conditions of CPFX have been considered. The CPFX concentration in the range of 1.0 × 10-6 - 4.0 × 10-5 M is linear to the fluorescence intensity, F450nm. The detection limit was achieved at 4.0 × 10-7 M CPFX. The CPFX in real samples was determined with satisfactory results.
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© 2004 by The Japan Society for Analytical Chemistry
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