Capillary Electrophoresis with Chemiluminescence Detection of Rutin and Chlorogenic Acid Based on Its Enhancing Effect for the Luminol-Ferricyanide System

Abstract

A capillary electrophoresis with chemiluminescence method has been developed for the determination of rutin and chlorogenic acid based on its enhancing effect on the luminol-ferricyanide system. Under the optimum conditions, the analytes could be separated within 5 min, and the detection limits of the proposed method were 0.22 µg/ml for rutin and 0.50 µg/ml for chlorogenic acid, respectively. The method was successfully applied to the analysis of rutin and chlorogenic acid in real samples.