Spectrofluorometric Determination of Trace Amounts of Coenzyme II Using Norfloxacin-Terbium Complex as a Fluorescent Probe

Abstract

When terbium ion (Tb³⁺)-norfloxacin (NFLX) complex is issued a fluorescent probe, in a buffer solution of pH = 7.6, NADP can remarkably enhance the fluorescence intensity of the Tb³⁺-NFLX complex at λ = 545 nm. The enhanced fluorescence intensity of Tb³⁺ is in proportion to the concentration of NADP. The dynamic range for the determination of NADP is 1.11 × 10^-7 - 6.16 × 10^-5 mol l^-1, with a detection limit of 4.31 × 10^-8 mol l^-1. This method is simple, practical and relatively free of interference from coexisting substances, so it can be successfully applied to determination of NADP in synthetic water samples.