Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Notes
Ultra-sensitive Colorimetric Method to Quantitate Hundreds of Polynucleotide Molecules by Gold Nanoparticles with Silver Enhancement
Bo-Sheng LILi-Fan ZHAOChi ZHANGXiao-Han HEIFang LIXiao-Bo LIJie SHENYuan-Yuan LIQiang HUANGShun-Qing XU
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2006 Volume 22 Issue 10 Pages 1367-1370

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Abstract

An ultra-sensitive colorimetric method to quantitate hundreds of polynucleotide molecules by gold nanoparticles with silver enhancement has been developed. The hybridization products from the target polynucleotides with biotin-labeled probes and gold nanoparticle-functioned oligonucleotides were immobilized to microplates via avidin-biotin system, and the absorbance signals of gold nanoparticles were amplified by silver enhance solution. This sandwich colorimetric assay can detect as few as 600 molecules for single-strand oligonucleotides and as few as 6000 molecules for double-strand polynucleotides in a 50 µL reaction system.

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© 2006 by The Japan Society for Analytical Chemistry
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