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Analytical Sciences
Vol. 22 (2006) No. 2 P 201-203

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http://doi.org/10.2116/analsci.22.201

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Enhancement of the Binding Ability of a Ligand for Nucleobase Recognition by Introducing a Methyl Group

Qing DAI1), Chun-Yan XU1), Yusuke SATO1), Keitaro YOSHIMOTO2), Seiichi NISHIZAWA1), Norio TERAMAE1)

1) Department of Chemistry, Graduate School of Science, Tohoku University, and CREST, Japan Science and Technology Agency (JST) 2) Bioengineering Laboratory, RIKEN (The Institute of Physical and Chemical Research)

Released 2006/03/24  
received 2005/12/05   accepted 2006/01/06

The recognition ability of pteridine derivatives for nucleobases opposite an abasic (AP) site in an oligodeoxynucleotide (ODN) duplex is enhanced by using a propylene residue (Spacer-C3) as an AP site. The recognition ability is further enhanced both by attaching methyl groups to a fluorescent ligand and by measuring the fluorescence response at 5°C; 6.2 × 106 M-1 of the binding constant is attained between 2-amino-6,7-dimethyl-4-hydroxypteridine and guanine opposite the AP site in water.

Copyright © 2006 by The Japan Society for Analytical Chemistry
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