Spectrophotometric Determination of Anthracycline Anticancer Agents with Aluminum(III) and Chromazurol S in a Nonionic Surfactant Micellar Medium

Abstract

A simple and highly sensitive spectrophotometric method for the determination of anthracycline anticancer agents, such as Daunorubicin hydrochloride (DAU), was established by using aluminum(III) and Chromazurol S (CAS) in a nonionic surfactant micellar medium. In the case of determination of DAU, the apparent molar absorptivity was 1.3 × 10⁵ dm³ mol^-1 cm^-1 at 615 nm. Beer's law was obeyed in the concentration range of 0.028 - 2.82 µg ml^-1 for DAU. Owing to no need for solvent extraction, this method could be applied to assays of DAU and related drugs in pharmaceutical preparations.