Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Notes
Optical Parameter Dependence of Fluorescence Correlation Spectrometry without Using Magnification Tools
Kitao FUJIWARAShun HIROKAWAMotohide AOKI
Author information
JOURNALS FREE ACCESS

2007 Volume 23 Issue 10 Pages 1237-1239

Details
Abstract

The performance of fluorescence correlation spectrometry (FCS) was examined for studying the solutions suspended with the fluorescent particles of various sizes from 50 nm to 10 µm in diameter and for different sizes of pinholes: the particles were made to move by simply stirring the solution in the quartz fluorescence cuvette. Without using any magnification tool for the optical image, this FCS system successfully distinguishes images with a size of smaller than 1 µm. This system was applied for determination of the sizes of microalgae.

Information related to the author
© 2007 by The Japan Society for Analytical Chemistry
Previous article Next article
feedback
Top