Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Quantitative Analysis of Epinephrine in Human Plasma Samples Using Kinetic Fluorometric Method Combined with Second-order Calibration
Shu-Fang LIHai-Long WUA-Lin XIAShao-Hua ZHUJin-Fang NIEYong-Jie YURu-Qin YU
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2009 Volume 25 Issue 10 Pages 1231-1236

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Abstract

A kinetic fluorometric method was proposed for the quantitative determination of epinephrine (EP) in human plasma samples with the aid of second-order calibration methods based on alternating trilinear decomposition (ATLD) and alternating fitting residue (AFR) algorithms. It was based on that EP could be gradually converted to a highly fluorescent intermediate product by an oxidation reaction, and further to a non-fluorescent degradation product (o-quinone). These methodologies fully exploit the second-order advantage of the employed three-way kinetic fluorescence data, allowing the concentrations of EP to be quantified even in the presence of uncalibrated interferences. The average recoveries obtained from ATLD and AFR with a factor number of 2 (N = 2) were 100.7 ± 3.3 and 100.4 ± 2.2%, respectively. In addition, elliptical joint confidence region (EJCR) tests as well as figures of merit (FOM) were employed to evaluate the accuracy of the two algorithms.

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© 2009 by The Japan Society for Analytical Chemistry
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