Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
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α-Glucosidase Inhibition Assay in an Enzyme-immobilized Amino-microplate
Toshiro MATSUIMayu SHIMADANozomi SAITOKiyoshi MATSUMOTO
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2009 Volume 25 Issue 4 Pages 559-562

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Abstract
α-Glucosidase (AGH) from the small intestine of rat was immobilized onto a glutaradehyde (GA) activated NH2-96 well microplate to establish a convenient and rapid AGH inhibition assay system. After AGH immobilization, remaining GA groups were blocked by β-alanine to induce a negative charge on the surface of the well. The AGH-plate showed an enzyme activity of 444 nU/well under an assayed condition at 37°C for 2 h using 0.3 mM 4-methylumbelliferyl-α-D-glucopyranoside as a fluorogenic substrate. Inhibitory powers of voglibose and acarbose as therapeutic AGH inhibitors were successfully evaluated to have IC50 values of 13 and 114 nM, respectively.
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© 2009 by The Japan Society for Analytical Chemistry
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