Simultaneous Flow-Injection Solid-Phase Fluorometric Determination of Thiabendazole and Metsulfuron Methyl Using Photochemical Derivatization

Abstract

A flow-through optosensor implemented with photochemically induced fluorescence (PIF) is reported for the simultaneous determination of thiabendazole (TBZ) and metsulfuron methyl (MET). TBZ is determined by measuring its native fluorescence once retained on the solid support filling the flow-cell of a FI-system. On the other hand, a strongly fluorescent photoproduct from MET is generated on-line in micellar medium by UV irradiation and monitored in a similar way to TBZ. MET photoproduct and TBZ are separated by placing in the flow-system a minicolumn, filled with C₁₈ silica gel, which allows their sequential arrival to the detection area. The sorption of the species on the solid support in the detection area provides a noticeable improvement in sensitivity and selectivity when comparing with their determination in homogeneous solution. The detection limits and RSDs for TBZ and MET are 2.5 and 3.3 ng ml^-1 and 1.1 and 2.4%, respectively. The method is successfully applied to environmental water samples.