Positive Charged Polymer as a Probe for DNA Determination by Resonance Light Scattering

Juan DU; Huzhi ZHENG; Chengzhi HUANG; Congying WEN; Jianyu HAO; Yufei HU; Dongbo ZHOU; Li LIU

doi:10.2116/analsci.25.727

Notes

Positive Charged Polymer as a Probe for DNA Determination by Resonance Light Scattering

Juan DU, Huzhi ZHENG, Chengzhi HUANG, Congying WEN, Jianyu HAO, Yufei HU, Dongbo ZHOU, Li LIU

Author information

Juan DU
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University
Huzhi ZHENG
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University
Chengzhi HUANG
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University
Congying WEN
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University
Jianyu HAO
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University
Yufei HU
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University
Dongbo ZHOU
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University
Li LIU
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University

JOURNAL FREE ACCESS

2009 Volume 25 Issue 5 Pages 727-730

DOI https://doi.org/10.2116/analsci.25.727

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Abstract

Electrostatic interactions of calf thymus DNA (ct-DNA) with a positive charged polymer were investigated based on measurements of enhanced resonance light scattering (RLS) signals, and were proved by scanning electron microscopy (SEM). The optimal pH value was 7.0 in a phosphate-buffered saline solution (PBS) and the maximum RLS peak located at 344.8 nm. The RLS signal enhancement (ΔI_RLS) could be linearly correlated with the concentration of ct-DNA in the range of 0.0900 to 2.70 µg mL^-1, and the detection limit (S/N = 3) was 17.0 ng mL^-1. The binding ratio and the binding constant between DNA and polymer were also provided.

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